Thermo Scientific™ Y-PER™ Yeast Protein Extraction Reagent

Release functionally active solubilized proteins with the first commercially available yeast lysis reagent to use a mild detergent lysis procedure.

Product Code	Brand	Quantity
11824121	Thermo Scientific™ 78991	200 mL
11814121	Thermo Scientific™ 78990	500 mL

Description

Thermo Scientific™ Y-PER Yeast Protein Extraction Reagent is the first commercially available yeast lysis reagent to use a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.

This detergent-based cell lysis buffer eliminates the need to use glass beads or mechanical disruption to break through the thick proteinaceous cell envelope to extract protein Y-PER Reagent is effective for S. cerevisiae and other popular species, making it applicable for use in fusion-tagged protein purification and reporter enzyme assays with these model organisms. The lysis reagent also can be used for genomic and plasmid DNA extraction from yeast. The standard protocol for protein extraction is easy: simply add an appropriate volume of Y-PER Reagent to pelleted yeast cells, incubate at room temperature for approximately 20 minutes and spin down the debris. The resulting supernatant is a concentrated protein solution, surpassing typical yields obtained with traditional glass bead disruption.

Highlights:

• More protein – extracts more than twice as much protein as glass bead methods

• Less hassle – eliminates the difficulties associated with traditional glass bead lysis (e.g., clinging static-charged beads, protein/bead clumps and runaway beads)

• Optmized for yeast – validated for use with Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris

• Versatile – effective for many different organisms, including Bacillus subtilus and both Gram-positive and Gram-negative bacteria; suitable for use in a diverse range of situations

Specifications

• Format: Liquid
• Product Type: Yeast Protein Extraction Reagent
• Content And Storage: Store product at room temperature.
• Product Line: Y-PER™

Safety and Handling

Product Identifier

• Y-PER Yeast Protein Extraction Reagent

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : glycerol, disodium hydrogenorthophosphate

For Research Use Only. Not for use in diagnostic procedures.