Invitrogen™ PureLink™ RNA Micro Scale Kit

PureLink™ technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification.

Brand:  Invitrogen™ 12183016

Product Code. 10184903

Description

A simple and reliable technology for everyday RNA purification
PureLink™ technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution. The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA. Purify RNA from up to 10 mg of tissue or 10⁵ cells. Clean up and concentrate RNA amounts ranging from pg to 100 μg in volumes as small as 10 μL. Purified RNA is ready for use in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA library construction, and nuclease protection assays.

A simple and rapid procedure
Homogenize your sample in lysis buffer, add 70% ethanol, and mix. Deposit the resulting solution into a PureLink Micro kit column with collection tube, and centrifuge for one minute. Nucleic acids bind to the PureLink silica membrane in the column, and after subsequent washes and on-column digestion with DNase, purified RNA can be eluted with RNase-free water.

• Effective: Expect excellent RNA recovery (up to 100 μg total RNA) from small samples
• Convenient and safe: The extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol
• Fast: Obtain high-quality total RNA, typically in just 15 minutes
• Reliable: Obtain ready-to-use RNA suitable for any downstream application

Specifications

• Content And Storage: Box 1 (PureLink™ RNA Micro Kit):
  • Lysis Buffer, 125 mL
  • Wash Buffer I, 50 mL
  • Wash Buffer II, 15 mL
  • RNase-free Water, 15.5 mL
  • PureLink™ Micro Kit Columns with collection tubes, 50 each
  • Collection Tubes, 50 each
  • Recovery Tubes, 50 each
  
  Box 2 (PureLink™ DNase/Carrier RNA):
  • Lyophilized PureLink™ DNase, 1500 Units
  • PureLink™ On-Column DNase 2X Buffer, 1 mL
  • RNase-free Water, 1 mL
  • Lyophilized PureLink™ Carrier RNA, 300 μg
  
  Store all components of Box 2 at 4°C. Store remaining components at room temperature.
• Purification Time: 15 min. (After sample homogenization)
• Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM), Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 17 μg

• Isolation Technology: Silica Spin Column
• Elution Volume: 12–22 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Scale: Micro
• Starting Material Amount: Cells: ≤10⁵
  Tissue: ≤5 mg
  Enzymatic Reactions: ≤1.2 mL

For Research Use Only. Not for use in diagnostic procedures.