Corning™ DMEM (Dulbecco's Modified Eagle's Medium)

Corning™ DMEM (Dulbecco's Modified Eagle's Medium) support cell culture growth and substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over original formulation. Products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis.

Brand:  Corning™ 15-013-LX

Product Code. 15313621

Description

• Modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME
• Original formulation was used to culture embryonic mouse cells
• Has been modified to support variety of normal and transformed cells, such as primary cultures of mouse and chicken cells
• Formulations can differ in amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine
• Tested to determine pH and osmolality
• Low levels of endotoxin, and absence of contamination
• Tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Chromogenic Assay
• Osmolality: 335±mOsm/kg H₂O
• Mycoplasma: Tested Negative
• Sterility: Pass

Specifications

• Content And Storage: 2 to 8°C
• Form: Liquid
• With Additives: Glucose, Sodium Pyruvate
• Endotoxin Level: <0.25EU/mL
• For Use With (Application): To assess growth promotion and cytotoxicity characteristics

• Concentration: 1 X
• Product Type: DMEM Media
• Without Additives: No Glutamine
• Formulation: Inorganic Salts: 200mg/L CaCl2 (anhydrous), 0.1mg/L Fe(NO3)3.9H2O, 400mg/L KCl, 97.7mg/L MgSO4 (anhydrous), 6400mg/L NaCl, 125mg/L NaH2PO4.H2O, 3700mg/L NaHCO3; Amino Acids: 84mg/L L-Arginine. HCl, 62.57mg/L L-Cystine.2HCl, 30mg/L Glycine, 42mg/L L-Histidine.HCl.H2O, 104.8mg/L L-Isoleucine, 104.8mg/L L-Leucine, 146.2mg/L L-Lysine. HCl, 30mg/L L-Methionine, 66mg/L L-Phenylalanine, 42mg/L L-Serine, 95.2mg/L L-Threonine, 16mg/L L-Tryptophan, 103.79mg/L L-Tyrosine.2Na.2H2O, 20mg/L L-Valine; Vitamins: 4mg/L D-Calcium pantothenate, 4mg/L Choline chloride, 4mg/L Folic Acid, 7.2mg/L i-Inositol, 4mg/L Nicotinamide, 4mg/L Pyridoxine. HCl, 0.4mg/L Riboflavin, 4mg/L Thiamine. HCl; Other: 4500mg/L D-Glucose, 15mg/L Phenol red. Na, 110mg/L Sodium pyruvate; Added: 584mg/L L-glutamine Powder, 20 mL/L 200 mM Solution.
• Quantity: 1 x 10 L