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Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO
A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules.
Brand: Thermo Scientific™ C20041
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Description
- A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications
- The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules
- Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner
- Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately)
- The coupling or crosslinking procedure is typically done in two steps:
- First, a protein or thiol containing molecule is labeled with a DBCO group and excess crosslinker is removed
- Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety
- Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst
- Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
Specifications
Azide-Sulfhydryl | |
Maleimide-PEG4-DBCO | |
Yes | |
Upon receipt store desiccated at -20°C. | |
Wet Ice | |
Chemical Labeling | |
Maleimide, DBCO | |
Solid | |
DMF, DMSO | |
No |
No | |
647.74 | |
29.7 Å | |
Not determined | |
EZ-Link™ No-Weigh™ | |
Heterobifunctional | |
Medium (10 to 30 Å) | |
10 x 1 mg | |
Standard, Single-use | |
Crosslinker |