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Fisher BioReagents™ Taq DNA Polymerase
Allows up to 100 cycles of amplification without loss of activity
£42.90 - £3655.00
Specifications
Content And Storage | -20°C |
---|---|
Description | Taq DNA Polymerase |
Form | Liquid |
For Use With (Application) | Standard PCR |
Concentration | 5U/μL |
Product Code | Brand | Composition | Quantity | Price | Quantity & Availability | ||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Composition | Quantity | Price | Quantity & Availability | ||||
10458923
|
Fisher BioReagents™
FB6000-30 |
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | 5 × 500 Units | N/A | |||||
10448733
|
Fisher BioReagents™
FB6000-10 |
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | 100 Units | N/A | |||||
10717185
|
Fisher BioReagents™
FB6000-20 |
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | 25 × 100 Units | N/A | |||||
10091263
|
Fisher BioReagents™
FB6000-15 |
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | 5 × 100 Units | N/A | |||||
10458733
|
Fisher BioReagents™
FB6000-25 |
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | 500 Units | N/A | |||||
10698393
|
Fisher BioReagents™
FB6000-35 |
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | 2500 Units | N/A | |||||
10144924
|
Fisher BioReagents™
FB6000-40 |
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial | 5 × 2500 Units | N/A | |||||
10449693
|
Fisher BioReagents™
FB6000-45 |
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial | 100 Units | N/A | |||||
10346363
|
Fisher BioReagents™
FB6000-50 |
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial | 5 × 100 Units | N/A | |||||
10216744
|
Fisher BioReagents™
FB6000-70 |
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial | 2500 Units | N/A | |||||
10356363
|
Fisher BioReagents™
FB6000-60 |
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial | 500 Units | N/A | |||||
10206744
|
Fisher BioReagents™
FB6000-55 |
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial | 25 × 100 Units | N/A | |||||
10104974
|
Fisher BioReagents™
FB6000-75 |
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial | 5 × 2500 Units |
£3655.00
Pack of 5 |
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10071553
|
Fisher BioReagents™
FB6000-65 |
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial | 5 × 500 Units | N/A | |||||
Description
- Licensed for use in PCR
- Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
- Has a 5«→3« exonuclease activity
- Available with two different reaction buffers
- Buffer A contains an optimized final concentration of 15mM MgCl2 for most PCR amplifications
- Buffer B does not contain MgCl2; MgCl2 is provided in a separate vial as a 25mM solution
- Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
- Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl2) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl2 solution in a separate vial
Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above
Specifications
-20°C | |
Liquid | |
5U/μL |
Taq DNA Polymerase | |
Standard PCR | |
Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5% |