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Thermo Scientific™ GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 Cells
GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells contain the human Complement Component 5a Receptor 1 (C5aR1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1746
Description
GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells contain the human Complement Component 5a Receptor 1 (C5aR1) (Accession No. NM_000710) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 (Cat. No. K1537) contains a beta-lactamase reporter gene under control of a NFAT response element and a promiscuous G Protein, Gα15, stably integrated into CHO-K1 cells.
The GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of C5a. In addition, GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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Specifications
NM_001736.2 | |
Dividing Cells | |
GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, FRET Technique, Liquid Nitrogen Storage | |
FRET | |
GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells, GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 Assay Protocol, Certificate of Analysis | |
GeneBLAzer™ | |
BLA (Beta-Lactamase) | |
C5AR1 | |
CHO-K1 | |
GPCR |
Cell-based beta lactamase reporter gene | |
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells • GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 Assay Protocol • Certificate of Analysis The GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
Fluorescence | |
C5aR1 | |
Single Cell Clone Origin | |
2 x 106 Cells | |
CHO-K1 | |
CHO-K1 Cells | |
GeneBLAzer™ |
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For Research Use Only. Not for use in diagnostic procedures.