Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

Brand:  Invitrogen™ 25530049

Product Code. 11588916

Description

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C
• Protein Filter: P

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Specifications

• Concentration: 20 mg/mL
• For Use With (Application): Chromatin Biology/RNA Extraction/DNA Extraction
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Product Type: Proteinase K

• Purity: DNase-Free, RNase-Free
• Content And Storage: Store in freezer (-5 to -30°C).
• Quantity: 5 mL
• Form: Liquid

Safety and Handling

Product Identifier

• 5 ML PROTEINASE K SOLUTION RNA STORE AT -20C

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1

Hazard Statement

• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Proteinase, tritirachium album serine

For Research Use Only. Not for use in diagnostic procedures.