Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Brand:  Thermo Scientific™ 22981

Product Code. 11861335

Description

Thermo Scientific™ Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.

EDC is the abbreviation for 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, a crosslinker that has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5Æphosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS, Part No. 24510), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.

Highlights:

• Zero-length crosslinker
• Numerous chemical conjugates can be synthesized via available –COOH and –NH2 groups
• Amide bond formed provides a neutral linkage, which is ideal for preparing peptides and antigens
• Adding Sulfo-NHS (Part No. 24510) enhances the coupling reaction at physiological pH conditions
• Reactive groups: carbodiimide
• Reactive toward: carboxyl and amino groups

Specifications

• Chemical Reactivity: Amine-Carboxyl
• Description: EDC
• PEGylated: No
• Content And Storage: Upon receipt store desiccated at -20°C.
• Shipping Condition: Wet Ice
• Labeling Method: Chemical Labeling
• Reactive Moiety: Carbodiimide
• Form: Powder
• Solubility: Water
• Water Soluble: Yes

• Cleavable: No
• Molecular Weight (g/mol): 191.7
• Spacer Arm Length: 0.0 Å
• Cell Permeability: No
• Product Line: Pierce™
• Crosslinker Type: Heterobifunctional
• Spacer: Short (<10 Å)
• Quantity: 25 g
• Format: Standard, Single-use, Premium-grade
• Product Type: Crosslinker

Safety and Handling

Product Identifier

• EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : N-(ethylcarbonimidoyl)-N,N-dimethyl-1,3-propanediamine monohydrochloride

For Research Use Only. Not for use in diagnostic procedures.