Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Product Code	Brand	Quantity
12391973	Thermo Scientific™ 88276	5 Columns
12201203	Thermo Scientific™ 88277	25 Columns

Description

Reduce endotoxin levels in protein samples by greater than 99% in one hour while maximizing protein recovery with Thermo Scientific™ Pierce High Capacity Endotoxin Removal Resin.

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(epsilon-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by greater than 99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers. Pierce High capacity Endotoxin Removal Resin is available as a slurry to pack a custom column that can be re-used at least 5 times with no loss in endotoxin removal efficiency. This resin is also available in a new single-use spin column format optimized for different sample volumes.

Highlights:

• High capacity bind up to 2,000,000 EU/mL to eliminate at least 99% of endotoxins

• Durable re-use resin up to 10 times with no loss in performance

• Selective recover at least 85% of your protein sample

• High performance compatible with FDA guidelines by reducing final EU concentration to less than 5 EU/mL

• Fast our new spin cup format enables endotoxin depletion within 1 hour

• Cleansingle-use spin columns avoid cross contamination of samples

• Optimized spin cups are optimized for different sample volumes

• Economical large-volume discounts available

Requires:

Endotoxin-free water

Recommended for:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

Specifications

• Capacity (Metric): Up to 2,000,000 EU/mL
• Content And Storage: Upon receipt store at 4°C.
• Product Line: Pierce™
• Type: Spin Column
• Format: Spin Column

• Formulation: 22mL-capacity centrifuge columns containing 1mL resin
• Form: Liquid
• Target: Endotoxin Removal
• Stationary Phase: Proprietary Ligand
• Column Type: Affinity, Cellulose Resin

Safety and Handling

Product Identifier

• Pierce High Capacity Endotoxin Removal Spin Columns, 1 mL

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Reproductive toxicity Category 1
• Skin corrosion/irritation Category 2

Hazard Statement

• H226-Flammable liquid and vapour.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H340-May cause genetic defects.
• H351-Suspected of causing cancer.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol

For Research Use Only. Not for use in diagnostic procedures.