Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this N-hydroxysuccinimide modification reagent.

Brand:  Thermo Scientific™ PG82071

Product Code. 15215763

Description

Optimize applications where product integrity and risk minimization are paramount with the specially characterized Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS, our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent.

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (–COOH) for conjugation with primary amines (–NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Highlights:

• Sulfo-NHS – use with EDC crosslinker to prepare amine-reactive NHS ester reagents
• High quality – identity and purity confirmed by several tests, including quantitative NMR
• Product integrity – enhanced level of testing and characterization compared to standard grade
• Lot retention – ample supply of past lots retained to ensure future process testing
• Change management – Change Control Notification (CCN) service
• Consistent manufacture – batch-specific manufacturing documentation review

Applications:

• Improve efficiency of EDC coupling reactions
• Convert carboxyls to amine-reactive sulfo-NHS esters
• Membrane-impermable for cell surface labeling
• Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
• Activate nanoparticles with amine-reactive sulfo-NHS esters

Specifications

• Chemical Reactivity: Amine-Carboxyl
• Description: Sulfo-NHS
• PEGylated: No
• Cell Permeability: No
• Product Line: Pierce™
• Crosslinker Type: Heterobifunctional
• Spacer: Short (<10 Å)
• Quantity: 500 mg
• Format: Standard, Single-use, Premium-grade
• Product Type: Crosslinker

• Cleavable: No
• Molecular Weight (g/mol): 217.13
• Content And Storage: Upon receipt store at 4°C.
• Shipping Condition: Ambient
• Labeling Method: Chemical Labeling
• Reactive Moiety: Carbodiimide
• Form: Powder
• Solubility: Water
• Water Soluble: Yes

Safety and Handling

Product Identifier

• Pierce Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H319-Causes serious eye irritation.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol

For Research Use Only. Not for use in diagnostic procedures.