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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Brand: Thermo Scientific™ 34047

189.65 GBP valid until 2025-12-31
Use promo code "25339" to get your promotional price.

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Product Code. 11859270

£203.00 / 105 tablets

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Description

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

The substrate is offered in several formats:
PNPP Substrate
• Powder Form

PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.

Specifications

Description	PNPP Tablets
Quantity	105 Tablets
Format	Tablet
Substrate Type	Alkaline Phosphatase
Shipping Condition	Wet Ice

Product Type	ELISA Substrate
Detection Method	Colorimetric
Substrate Properties	Chemical Substrate
Content And Storage	Upon receipt store product at -20°C.

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Documents

Scientific Resources

Application Notes

Tech Tip: Guide to enzyme substrates for ELISA applications File size: 53.4 kB

Product Information

Manuals

User Guide: PNPP − Phosphatase Substrate Version: B.0 File size: 72.3 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZJ403603	Certificate TypeCertificate of Analysis	Date16/12/2024	Catalog Number34047
Lot NumberZI403602	Certificate TypeCertificate of Analysis	Date15/10/2024	Catalog Number34047
Lot NumberZH402237	Certificate TypeCertificate of Analysis	Date13/09/2024	Catalog Number34047
Lot NumberZH402236	Certificate TypeCertificate of Analysis	Date30/08/2024	Catalog Number34047
Lot NumberZH394053	Certificate TypeCertificate of Analysis	Date20/08/2024	Catalog Number34047

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