Life Science Buffers
![](https://assets.fishersci.com/TFS-Assets/CCG/product-images/life-science-buffers-header-image.jpg-250.jpg)
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (61)
- (1)
- (1)
- (111)
- (2)
- (2)
- (5)
- (10)
- (1)
- (152)
- (8)
- (1)
- (1)
- (2)
- (101)
- (2)
- (442)
- (1)
- (12)
- (5)
- (6)
- (2)
- (2)
- (2)
- (2)
- (4)
- (12)
- (22)
- (42)
- (7)
- (3)
- (51)
- (7)
- (3)
- (3)
- (25)
- (14)
- (54)
- (1)
- (12)
- (3)
- (1)
- (10)
- (1)
- (52)
- (3)
- (1)
- (3)
- (26)
- (5)
- (4)
- (2)
- (28)
- (14)
- (4)
- (75)
- (6)
- (18)
- (19)
- (15)
- (8)
- (7)
- (8)
- (8)
- (34)
- (8)
- (39)
- (10)
- (8)
- (3)
- (5)
- (35)
- (4)
- (6)
- (4)
- (7)
- (2)
- (4)
- (12)
- (28)
- (2)
- (1)
- (1)
- (3)
- (4)
- (4)
- (1)
- (5)
- (102)
- (148)
- (2)
- (3)
- (6)
- (10)
- (75)
- (17)
- (1)
- (5)
- (2)
- (2)
- (1)
- (16)
Filtered Search Results
![](/catalog/search/static/resources/images/icons/back_to_top_icon.png)
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
![GREENER_CHOICE](/content/dam/fishersci/glyphs/green_glyph.png)
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
PBS (10X), pH 7.4
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.3 to 7.5 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3100 mOsm/kg |
Gibco™ PBS (10X), pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
![GREENER_CHOICE](/content/dam/fishersci/glyphs/green_glyph.png)
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
PBS (10X), pH 7.2
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.1 to 7.3 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3200 mOsm/kg |
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Room Temperature |
---|---|
Dilution | 10 X |
Content And Storage | Store at room temperature. |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.4 |
Product Line | Ambion™ |
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
![GREENER_CHOICE](/content/dam/fishersci/glyphs/green_glyph.png)
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Ambient |
---|---|
Dilution | 1 X |
Content And Storage | 15°C to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.3 to 7.5 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
Dilution | 10 X |
---|---|
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Color | Colorless |
pH | 7.4 |
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
![GREENER_CHOICE](/content/dam/fishersci/glyphs/green_glyph.png)
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Dilution | 1 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
Thermo Scientific Chemicals TRIS, 0.5M buffer soln., pH 6.8
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00004679 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 6.0
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
PubChem CID | 16218417 |
---|---|
CAS | 145224-94-8 |
Molecular Weight (g/mol) | 213.248 |
MDL Number | MFCD00283848 |
SMILES | C1COCCN1CCS(=O)(=O)O.O |
IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
Molecular Formula | C6H15NO5S |
TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Boiling Point | 100°C |
---|---|
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 7.5
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
PubChem CID | 23831 |
---|---|
CAS | 7365-45-9 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
MDL Number | MFCD00006158 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
Molecular Formula | C8H18N2O4S |
Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 8.0
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
PubChem CID | 23831 |
---|---|
CAS | 7365-45-9 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
MDL Number | MFCD00006158 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
Molecular Formula | C8H18N2O4S |
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
![EDGE](/content/dam/fishersci/glyphs/edge.png)
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00132476 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |