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General Western Blot Reagents

General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 6 of 6 results
1

Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

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Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility PVDF, Nitrocellulose
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Product Line Invitrogen™
2

Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent

Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane

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Product Type ECL Western Blotting Detection Reagent
Storage Requirements 18°C to 30°C
For Use With (Application) Routine and confirmatory Western blotting
3

Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane

Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.

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EDGE
4

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-goat

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

5

Invitrogen™ Novex™ Reversible Membrane Protein Stain Kit

Used for determining protein transfer efficiency after blotting

6

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Product Type Transfer Buffer