Flow Cytometry Compensation, Counting and Calibration
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Flow Cytometry Compensation, Counting and Calibration
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Molecular Probes™ CountBright™ Absolute Counting Beads, for flow cytometry
CountBright™ Absolute Counting Beads, for flow cytometry
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Invitrogen™ ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits)
ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits)
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Shipping Condition | Room Temperature |
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Format | Tube |
For Use With (Equipment) | Flow Cytometer |
Product Type | Compensation Beads |
Diameter (Metric) | 6 μm |
Stain Configuration | Surface |
Quantity | 1 Kit |
Detection Method | Fluorescence |
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
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Product Type | Cell Stimulation Cocktail |
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Product Line | eBioscience™ |
Invitrogen™ eBioscience™ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
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Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer
A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.
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Product Type | Staining Buffer |
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Product Line | eBioscience™ |
Invitrogen™ eBioscience™ IC Fixation Buffer
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
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Invitrogen™ eBioscience™ Fixation/Permeabilization Diluent
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
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Invitrogen™ UltraComp eBeads™ Compensation Beads
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
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For Use With (Equipment) | Flow Cytometer |
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Product Type | Compensation Beads |
Product Line | eBeads™ |
Invitrogen™ eBioscience™ Fixation/Permeabilization Concentrate
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
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Invitrogen™ 123count eBeads™ Counting Beads
Intended for use in absolute counting of cells or other particles by flow cytometry. 123count eBeads are 7μm microparticles containing encapsulated dyes compatible with blue (488nm) and violet (405nm) excitation sources and emitting fluorescence between approximately 500nm and 750nm.
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Invitrogen™ CountBright™ Plus Absolute Counting Beads
Calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.
Invitrogen™ AbC™ Total Antibody Compensation Bead Kit
Provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry.
Shipping Condition | Room Temperature |
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Content And Storage | Contains 1 vial AbC™ Total Compensation capture beads and 1 vial of negative beads. |
Format | Tube |
For Use With (Equipment) | Flow Cytometer |
Product Type | Compensation Beads |
Diameter (Metric) | 6 μm |
Excitation Wavelength Range | any lasers (UV to 633/635) |
Product Line | AbC™ |
Detection Method | Fluorescence |
Invitrogen™ Cell Sorting Set-up Beads (for blue lasers)
Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.
Molecular Probes™ Flow Cytometry Size Calibration Kit (nonfluorescent microspheres)
Flow Cytometry Size Calibration Kit (nonfluorescent microspheres)
Shipping Condition | Room Temperature |
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Content And Storage | Store at room temperature. |
Product Type | Fixation Medium |
Reagent Type | Fixation Reagent |