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LAMP and Other Isothermal Amplification Technologies

LAMP and Other Isothermal Amplification Technologies

Nucleic acid (RNA and DNA) amplification products designed to amplify targeted material at a constant temperature as opposed to thermocylcing. Products include loop-mediated isothermal amplification (LAMP) assays as well as other isothermal amplification reagents, kits, and buffers. Products are well suited for detecting a variety of pathogens from a broad array of source material, including animal, plant, and environmental samples.
Isothermal Amplification Reagents (14)
LAMP Assays (2)

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1 – 8 of 8 results
1

Thermo Scientific™ EquiPhi29™ DNA Polymerase

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.

Promotions are available
Shipping Condition Dry Ice
Polymerase EquiPhi29 DNA Polymerase
Product Type Stand-alone enzyme
Reaction Time 2 hours
Concentration 10 U/μL
Optimal Reaction Temperature 42°C
For Use With (Application) MDA-WGA,RCA
2

Invitrogen™ Lyo-ready Bst DNA Polymerase

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.

Shipping Condition Dry Ice
Polymerase Lyo-ready Bst DNA Polymerase
Product Type Stand-alone enzyme
Reaction Time ≤10 minutes
Optimal Reaction Temperature 65°C
For Use With (Application) LAMP,RT-LAMP
3

Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)

Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.

EDGE
Shipping Condition Dry Ice
Polymerase phi29 DNA Polymerase
Product Type Stand-alone enzyme
Reaction Time 4 hours
Concentration 10 U/μL
Optimal Reaction Temperature 30°C
For Use With (Application) MDA-WGA,RCA
4

Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)

Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.

5

Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.

6

Invitrogen™ SuperScript™ IV RT-LAMP Master Mix

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.

Shipping Condition Dry Ice
Polymerase Bst DNA Polymerase
Includes Label or Dye SYTO 9 Green Fluorescent Nucleic Acid Stain
Reverse Transcriptase SuperScript IV
Product Type Master Mix
Reaction Time 5 to 15 minutes
Concentration 2X
Optimal Reaction Temperature 65°C
For Use With (Application) LAMP,RT-LAMP
7

Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2

The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.

Includes LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA
Detection Limit <250 copies/25 μL (Direct LAMP)
<100 copies/25 μL (Purified RNA-based LAMP)
Bead Type Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads
For Use With (Equipment) Heating block
Enzyme SuperScript IV & proprietary Bst-derived DNA polymerase
Formulation Liquid. 5 components included in the kit.
Sample Type Saliva or Nasopharyngeal/Nasal Swab
Color During reaction color changes from purple to blue
Sample Volume Range of sample inputs
Key Functions Detects SARS-CoV-2 presence
For Use With (Application) SARS-CoV-2 surveilance
Detection Method Colorimetric readout
8

Thermo Scientific™ EquiPhi29™ DNA Amplification Kit

The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

Shipping Condition Dry Ice
Polymerase EquiPhi29 DNA Polymerase
Format Kit
Form Liquid
Product Type DNA Amplification Kit
Reaction Time Up to 2 hours
Research Category Nucleic Acid Analysis,In Vitro Transcription,Cell-free Protein Expression,In Situ Sequencing,SNP Detection
Optimal Reaction Temperature 42°C
For Use With (Application) RCA,MDA-WGA