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Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein A covalently bound to Dynabeads |
| Surface Functionality | Protein A |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein A |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Gibco™ Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation
Dynabeads Human T-Activator CD3/CD28 beads are 4.5-μm superparamagnetic beads covalently coupled with an optimized combination of anti-human CD3 and anti-human CD28 antibodies and intended for ex vivo activation and expansion/proliferation of human T cells.
| Shipping Condition | Ambient Temperature |
|---|---|
| Clonality | Monoclonal |
| Target Species | Human T-cells |
| Host Species | Mouse |
| Surface Functionality | Epoxy |
| Isolation Technology | Magnetic Beads |
| Material | Magnetic Polystyrene |
| Concentration | 4 x 10 7 Beads/mL |
| For Use With (Application) | Activation and Expansion of T-cells |
| Regulatory Status | For Research Use Only |
| Reactivity | Human |
| For Use With (Equipment) | DynaMag™ Magnet |
| Product Type | Antibody Coated Bead |
| Diameter (Metric) | 4.5 μm |
| Shelf Life | 24 months from date of manufacture |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 4.5 μm (CV <5%) |
| Product Line | DynaBeads |
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Thermo Scientific™ IPTG Solution, ready-to-use
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A
Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.
| Assay | BCA Assay |
|---|---|
| Specificity | Not Target-Specific |
| Content And Storage | Store at room temperature. |
| Description | Pierce BCA Protein Assay Reagent A |
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Product Type | Protein Quantitation Assay |
| For Use With (Application) | Solution-based Detection, Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Thermo Scientific™ RNA Gel Loading Dye (2X)
Prepare RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels using Thermo Scientific™ 2X RNA Loading Dye.
Thermo Scientific™ Pierce™ Bovine Serum Albumin Standard Pre-Diluted Set
High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
Invitrogen™ IPTG
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.
Thermo Scientific™ EDTA (0.5 M), pH 8.0
Optimize biochemistry or molecular biology applications that require a chelator for divalent cations.
Thermo Scientific™ Micro BCA™ Reagent A (MA)
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Thermo Scientific™ Micro BCA™ Reagent B (MB)
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 488 |
| Emission | 517 nm |
| Excitation | 494 nm |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor |
Poloxamer 188
Molecular Formula: C5H10O2 Synonym: Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer
| Synonym | Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer |
|---|---|
| Molecular Formula | C5H10O2 |
Invitrogen™ Wheat Germ Agglutinin (WGA)
Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) and Alexa Fluor, Alexa Fluor Plus, and other dyes. Fluorescent WGA conjugates bind to carbohydrates and are used for various cell biology applications such as plasma membrane labeling and cell painting assays.
| Ligand Type | N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues |
|---|---|
| Protein Family | Lectins |
| Protein Form | Heterodimer |
| Expression System | Wheat germ |
| For Use With (Application) | Flow Cytometry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Cell Painting |
| Protein Tag | None |
| Species | Wheat |
| Protein | Fluorescent lectins |
| Protein Subtype | Agglutinin |
Invitrogen™ iBind™ Flex Fluorescent Detection (FD) Solution Kit
Detection kit for use with iBind™ Flex Western System