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Search results for "cloning"

1 – 15 of 96,798 results
1

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Promotions are available
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
2

Bel-Art™ Cloning Cylinders

Manufactured from polystyrene. SP Scienceware™ Cloning Cylinders facilitates the isolation of individual cell clones.

Promotions are available
Product Type Cloning Cylinder
Material Polystyrene
Sterility Sterile
3

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

Promotions are available
4

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Promotions are available
EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO™
5

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Promotions are available
EDGE
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
6

Corning™ PYREX™ Cloning Cylinders
GREENER_CHOICE

Create identical cells by surrounding a single cell or group of cells

Product Type Cloning Cylinder
Material Borosilicate Glass
Sterility Non-sterile
7

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Promotions are available
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
8

Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells

An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.

Promotions are available
Content And Storage Dry Ice (-80°C)
Description Bacterial/Yeast Strain: DH10B
For Use With (Application) Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS)
9

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Promotions are available
Promoter T7
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type Cloning Kit
Cloning Method TA Cloning
Bacterial or Yeast Strain Not Included
For Use With (Application) PCR Cloning
Vector pCR2.1
Product Line TA Cloning™
10

Invitrogen™ Zero Blunt™ PCR Cloning Kit

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Promotions are available
Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain TOP10
Vector Blunt DNA Cloning Vectors
Product Line Zero Blunt™
11

Thermo Scientific™ pENTR™/D-TOPO™ Cloning Kit

Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System

12

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, without competent cells

Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products

Promotions are available
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter T3, T7
Content And Storage Box 1: Store at -5 to -30°C.
• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2: Store at room temperature.
• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method Zero Blunt TOPO
Bacterial or Yeast Strain Not Included
For Use With (Application) PCR Cloning
Vector pCR-XL-2-TOPO
Product Line TOPO™
13

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Promotions are available
14

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

15

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System