Invitrogen™ MEGAclear™ Transcription Clean-Up Kit

Quickly cleanup large-scale transcription reactions

Brand:  Invitrogen™ AM1908

Product Code. 10600226

Description

MEGAclear Kit is designed for quick cleanup of any large-scale transcription reaction. MEGAclear Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
  High recovery from 1ng to 500μg of input
  Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
  Quick protocol—purify RNA in less than 30 minutes
• Using the MEGAclear Kit: Protocol is quick and easy. Transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.

Specifications

• Content And Storage: Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
• Format: Tube
• Purification Target: mRNA, Synthetic mRNA
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: Ambion™, MEGAclear™
• Quantity: 20 Preps
• Shipping Condition: Room Temperature
• Yield: 500 μg

• Final Product Type: Purified Synthetic mRNA, Purified mRNA
• Isolation Technology: Silica Spin Column
• Sample Type: mRNA, Synthetic mRNA
• For Use With (Equipment): Vacuum Manifold, Centrifuge
• No. of Reactions: 20 Preps
• Product Type: mRNA purification kit, Purification kit
• Purification Time: 30 min
• Starting Material Volume: 1 ng to 500 μg of dsRNA>200b or ssRA>100bp

Safety and Handling

Product Identifier

• MEGAclear Transcription Clean-Up Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Skin corrosion/irritation Category 1 C

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 2-Mercaptoethanol

For Research Use Only. Not for use in diagnostic procedures.