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Invitrogen™ TURBO DNA-free™ Kit
Ideal tool for clearing residual DNA
Brand: Invitrogen™ AM1907
103.33 GBP valid until 2024-12-14
Use promo code "21632" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price
Includes: TURBO DNase, 10X TURBO DNase Buffer, DNase Inactivation Reagent, Nuclease-free Water
Description
Kit contains reagents for efficient, complete digestion of DNA along with removal of enzyme and divalent cations post-digestion
- Hyperactive TURBO DNase is catalytically superior enzyme compared to wild type DNase I
- Removes trace quantities of DNA that can interfere with RT-PCR
- Reagent included to completely remove DNase without phenol treatment or heating
- Method for genomic DNA removal prior to RT-PCR
- TURBO DNase is recombinant, engineered form of DNase I that is efficient than wild type DNase I in digesting away trace amounts of unwanted DNA
- TURBO DNase binds DNA substrates 6-fold more tightly than traditional DNase I, making enzyme tool of choice for clearing residual DNA that can generate a false positive signal in RT-PCR applications
- TURBO DNase now includes an enhancer that increases effectiveness by two orders of magnitude
- Efficient DNase and divalent cation removal without organic extraction or precipitation
- Conventional DNase treatment of RNA samples prior to RT-PCR typically call for inactivation of the DNase by phenol:CHCl3 extraction or heating followed by precipitation step to concentrate the RNA
- Phenol:CHCl3 extractions can be cumbersome and time-consuming
- Heating the sample to inactivate DNase can lead to chemical degradation of RNA by divalent cations present in DNase buffer
- TURBO DNA-free Kit circumvents problems using novel DNase Inactivation Reagent
- For removing the TURBO DNase from reaction, Inactivation Reagent also binds and removes divalent cations from TURBO DNase Reaction Buffer
PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription
Order Info
Shipping Conditions: Dry ice
Specifications
| Contains: TURBO DNase 10X TURBO DNase Buffer DNase Inactivation Reagent Nuclease-free Water Store components at -20°C (Nuclease-free water may be stored at room temperature) |
|
| DNase | |
| 50 Reactions | |
| Ambion™, DNA-free™ |
| Dry Ice | |
| Reaction Buffer | |
| TURBO DNA Free Kit |
For Research Use Only. Not for use in diagnostic procedures.