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Gibco™ Medium 199, no phenol red

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Brand: Gibco™ 11043023

Product Code. 11510386

£49.56 / 500mL

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:

With: L-glutamine

Without: Phenol Red, HEPES

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Product Use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco Medium 199 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Content And Storage	Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture.
Cell Line	Rat epithelial
Classification	Animal Origin
Form	Liquid
Sterility	Sterile-filtered
Without Additives	No Phenol Red, No Sodium Pyruvate, No HEPES
Osmolality	270 - 310 mOsm/kg
Quantity	500 mL

Culture Environment	CO₂
Cell Type	Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
Concentration	1 X
Product Type	Medium 199
With Additives	Glutamine, Sodium Bicarbonate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco™
Shipping Condition	Room Temperature

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