Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Brand:  Thermo Scientific™ A35391

Product Code. 15781819

Description

Thermo Scientific™ Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.

Thermo Scientific No-Weigh products are specialty reagents provided in pre-aliquoted format. The pre-weighed packaging helps prevent loss of reagent reactivity and contamination over time by minimizing repetitive opening and closing of vial. The format enables use of fresh vial of reagent each time, eliminating hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

• Reactive group: Carbodiimide
• Reaction target: Activates carboxyl groups to conjugate to amino groups (primary amines)
• Several conjugation strategies: React EDC alone with target groups or include NHS or Sulfo-NHS to increase reaction efficiency or to stabilize active intermediate for later reaction to amines
• Neutral linkage: Forms neutral amide bonds between carboxyls and amines
• Water-soluble Reagent: Add directly to reactions in aqueous, physiological buffers
• Soluble reaction byproducts: Easily removed by washing with water or dilute acid
• High-purity, crystalline reagent: Use to create high-quality, activated derivatives

Applications:

• Conjugate carboxyl and amino groups among peptides and proteins
• Couple haptens to immunogenic carrier proteins (e.g., attach a peptide to KLH)
• Immobilize peptide antigens to affinity-purify antibodies
• Create NHS-activated, amine-reactive labeling compounds
• Crosslink proteins to carboxyl coated beads or surfaces
• Activate nanoparticles with amine-reactive Sulfo-NHS esters

Specifications

• Chemical Reactivity: Amine-Carboxyl
• Description: EDC
• PEGylated: No
• Content And Storage: Upon receipt store desiccated at -20°C.
• Shipping Condition: Wet Ice
• Labeling Method: Chemical Labeling
• Reactive Moiety: Carbodiimide
• Form: Powder
• Solubility: Water
• Water Soluble: Yes

• Cleavable: No
• Molecular Weight (g/mol): 191.7
• Spacer Arm Length: 0.0 Å
• Cell Permeability: No
• Product Line: No-Weigh™, Pierce™
• Crosslinker Type: Heterobifunctional
• Spacer: Short (<10 Å)
• Quantity: 10 x 1 mg
• Format: Standard, Single-use, Premium-grade
• Product Type: Crosslinker

Safety and Handling

Product Identifier

• Pierce EDC No-Weigh, 10 x 1 mg

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : N-dimethyl-N-(ethylcarbonimidoyl)-N-1,3-propanediamine

For Research Use Only. Not for use in diagnostic procedures.