TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Brand:  TaqMan® 4351372

Product Code. 10330545

Description

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features of TaqMan Gene Expression assays include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Extensive content—Over 1.8 million predesigned assays available for over 25 different species
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
• Fast delivery—your assays shipped in as little as 1–2 days (see below)

Approximate ship time
• Inventoried: 1–2 days in North America, 3–5 days in Europe*
• Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.**

Recommended master mix: TaqMan Fast Advanced Master Mix (Cat. No. 4444556)

*Our most popular assays are stocked in North America and Europe for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.

**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

Specifications

• Content And Storage: 1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).
  
  Store at -15°C to -25°C.
  
  
• Form: Liquid
• PCR Method: qPCR
• For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
• Internal Probe Modification: FAM (5'), MGB (Minor Groove Binder) (3')
• Product Line: TaqMan™
• Quantity: S (360 reactions/360 μL), made to order
• Species: C. elegans, Cattle/Bovine, Dog/Canine, Drosophila, Horse/Equine, Swine/Porcine, Chicken, Zebrafish, Horse, Arabidopsis, Rabbit, Porcine, Rat, Human, Monkey, Mouse
• Fidelity (vs. Taq): 60 X

• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Green Features: Sustainable packaging
• Label or Dye: FAM
• Product Type: Gene Expression Assay
• Shipping Condition: Room Temperature
• Concentration: 20X
• No. of Reactions: S (360 x 360 μL Reactions), made to order

Safety and Handling

Product Identifier

• TaqMan Gene Expression Assay (FAM)

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 2
• Reproductive toxicity Category 1B
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H351-Suspected of causing cancer.
• H360-May damage fertility or the unborn child.
• H373-May cause damage to organs.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P314-Get medical advice/attention if you feel unwell.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : formamide

For Research Use Only. Not for use in diagnostic procedures.