Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (1)
- (1)
- (95)
- (5)
- (55)
- (1)
- (1)
- (1)
- (5)
- (3)
- (2)
- (1)
- (2)
- (107)
- (1)
- (122)
- (1)
- (2)
- (2)
- (1)
- (9)
- (1)
- (1)
- (20)
- (5)
- (16)
- (4)
- (1)
- (8)
- (1)
- (40)
- (3)
- (8)
- (31)
- (167)
- (28)
- (2)
- (3)
- (1)
- (12)
- (21)
- (1)
- (9)
- (7)
- (70)
- (135)
- (1)
- (4)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (86)
- (3)
- (3)
- (3)
- (7)
- (3)
- (5)
- (580)
- (5)
- (4)
- (4)
- (68)
- (27)
- (12)
- (21)
- (8)
- (4)
- (128)
- (6)
- (6)
- (18)
- (30)
- (2)
- (34)
- (19)
- (23)
- (2)
- (2)
- (8)
- (5)
- (14)
- (16)
- (54)
- (8)
- (45)
- (4)
- (3)
- (15)
- (8)
- (4)
- (2)
- (3)
- (3)
- (5)
- (6)
- (68)
- (4)
- (3)
- (4)
- (6)
- (5)
- (10)
- (3)
- (12)
- (4)
- (4)
- (12)
- (37)
- (2)
- (3)
- (1)
- (4)
- (2)
- (1)
- (2)
- (3)
- (3)
- (6)
- (3)
- (4)
- (1)
- (5)
- (6)
- (5)
- (2)
- (6)
- (1)
- (1)
- (9)
- (2)
- (3)
- (2)
- (2)
- (2)
- (3)
- (7)
- (1)
- (2)
- (4)
- (2)
- (2)
- (1)
- (2)
- (2)
- (3)
- (3)
- (1)
- (2)
- (1)
- (3)
- (3)
- (3)
- (15)
- (4)
- (1)
- (4)
- (3)
- (2)
- (2)
- (2)
- (4)
- (2)
- (1)
- (5)
- (12)
- (22)
- (42)
- (2)
- (9)
- (4)
- (1)
- (51)
- (7)
- (6)
- (5)
- (28)
- (14)
- (54)
- (1)
- (12)
- (2)
- (3)
- (1)
- (11)
- (1)
- (52)
- (3)
- (8)
- (1)
- (1)
- (3)
- (1)
- (1)
- (1)
- (3)
- (1)
- (26)
- (6)
- (4)
- (2)
- (2)
- (28)
- (3)
- (14)
- (4)
- (5)
- (105)
- (1)
- (153)
- (2)
- (3)
- (6)
- (14)
- (80)
- (2)
- (46)
- (2)
- (3)
- (22)
- (2)
- (16)
- (1)
- (5)
- (1)
- (4)
- (19)
- (2)
Filtered Search Results
Gibco™ DPBS, no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
DPBS, no calcium, no magnesium
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | DPBS |
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
PBS (10X), pH 7.4
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.3 to 7.5 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3100 mOsm/kg |
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Ambient |
---|---|
Dilution | 1 X |
Content And Storage | 15°C to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.3 to 7.5 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Fisher BioReagents™ Phosphate Buffered Saline Tablet
Each tablet prepares 100mL of 1X PBS solution. Fisher Bioreagents™ Phosphate Buffered Saline tablets (PBS) are a convenient option for making 1X phosphate buffered saline.
Gibco™ PBS, pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
PBS, pH 7.2
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 280 to 320 mOsm/kg |
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Gibco™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
DPBS, calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 1 X |
Content And Storage | 2° to 8°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 7 to 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Gibco™ DPBS, no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Form | Liquid |
---|---|
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.3 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 270 - 300 mOsm/kg |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Dilution | 1 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
Gibco™ PBS, pH 7.4 (flow cytometry grade)
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of applications, including reagent preparation, diluting cells for flow cytometry and as a cell culture reagent.
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
Form | Liquid |
Color | White |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2800 - 3100 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture |
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2630 - 3000 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 6.7 - 7.0 |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Gibco™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Dilution | 1 X |
---|---|
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.2 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Washing Cells Before Dissociation, Transporting Cells or Tissue Samples, Diluting Cells for Counting, and Preparing Reagents |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Packaging Type | Poly Cube |
pH | 7.3 to 7.5 |
Melting Point | 0°C |