PCR Equipment and Supplies
PCR Equipment and Supplies
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Cytiva Taq DNA Polymerase (cloned)
For PCR and other procedures requiring DNA polymerase activity at elevated temperatures
Content And Storage | 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl. Store at -20°C |
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Purity or Quality Grade | Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities. |
pH | 7.5 |
Concentration | 5000U/mL |
For Use With (Application) | Standard PCR |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Reaction Format | Separate Components |
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Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
For Use With (Application) | Cloning |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
AmpliTaq Gold™ 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
Shipping Condition | Room Temp or Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Green Features | Sustainable packaging |
Concentration | 2X |
For Use With (Application) | Cloning,Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold 360 DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.
Reverse Transcriptase | Maxima H Minus |
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Reaction Format | Separate Components |
Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
Sample Type | RNA |
For Use With (Application) | Cloning |
Product Line | Maxima |
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Purity or Quality Grade | DHPLC |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
Shipping Condition | Room Temperature |
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Separation Range | 100 bp to 12 kb |
Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
Starting Material Volume | 125 μL |
Isolation Technology | Silica Spin Column |
Elution Volume Max | 20 μL |
For Use With (Application) | Cloning,Standard PCR |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Target | DNA from Enzymatic Reactions (e.g. PCR) |
Format | Spin Column |
Product Type | PCR Micro Kit |
Run Time | 10 min. |
Elution Volume Min | 5 μL |
Product Line | PureLink™ |
Thermo Scientific™ Phire Green Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | Separate Components |
Product Type | Hot Start High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ 360 Buffer Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The AmpliTaq Gold™ 360 Buffer Kit is used in conjunction with AmpliTaq Gold™ 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient.
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Shipping Condition | Room Temperature |
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Format | Kit |
Sample Type | PCR Products |
Isolation Technology | Silica Spin Column |
Run Time | 10 min. |
Thermo Scientific™ Phire Green Hot Start II PCR Master Mix
Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ Phire Hot Start II PCR Master Mix
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix
The Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 2X |
For Use With (Application) | Cloning,Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold DNA Polymerase |
Size (Final Product) | 1.5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | Fast PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Invitrogen™ PureLink™ Pro 96 PCR Purification Kit
The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.