Test
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.
Content And Storage | Room temperature (18 - 25 °C) |
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Final Product Type | Transfection-grade plasmid DNA |
Size | < 15 kbp |
Isolation Technology | Silica Membrane and Endotoxin-reduction Technology |
Purity | A260/A280: 1.80 - 1.85 |
Sample Volume | Up to 5 mL |
For Use With (Application) | Rapid purification of plasmids for transfection |
Test Time | 14 min/6 preps |
Includes | NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A |
Format | Mini Spin Column |
For Use With (Equipment) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Efficiency | Binding capacity: 35 μg |
Sample Type | Bacterial Culture (E. coli, High-copy Plasmids) |
Yield | 15 - 30 μg |
Shelf Life | At least 1 year |
Elution Volume | Elution Volume: 30 - 50 μL |
Bacterial Endotoxins | <50 EU/ng DNA |
For expression of hROR gamma protein in mammalian cells, induction of IL-17A promoter activity
Yeast Rapid Pure Miniprep Plasmid Isolation Kit designed for yeast cells. Can isolate plasmid from bacterial transformants for use in any downstream application (PCR, sequencing, restriction enzyme digestion, labeling, etc).
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.
Content And Storage | Room temperature (18 - 25 °C) |
---|---|
Final Product Type | Transfection-grade plasmid DNA |
Size | < 15 kbp |
Isolation Technology | Silica Membrane and Endotoxin-reduction Technology |
Purity | A260/A280: 1.80 - 1.85 |
Sample Volume | Up to 5 mL |
For Use With (Application) | Rapid purification of plasmids for transfection |
Test Time | 45 min/plate |
Format | 96-well Plate |
For Use With (Equipment) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Efficiency | Binding capacity: 20 μg |
Sample Type | Bacterial Culture (E. coli, High-copy Plasmids) |
Yield | 5 - 20 μg |
Shelf Life | At least 1 year |
Elution Volume | Elution Volume: 100 - 200 μL |
Bacterial Endotoxins | <50 EU/ng DNA |
Nuclear Actin-Chromobody™ fused to TagGFP2.
For expression of hROR alpha protein in mammalian cells, induction of G6Pase promoter activity
For expression of hROR gamma (t) [del. 5-8] protein, which is a novel hROR gamma (t) isoform
For expression of hRUNX1 protein in mammalian cells, induction of FOXP3 promoter activity