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Protein Purification Reagents and Kits

Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 1,101 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Promotions are available
EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Promotions are available
EDGE
3

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Promotions are available
EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur™
4

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Promotions are available
EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
5

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

EDGE
6

Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

Promotions are available
EDGE
Type Agarose
Stationary Phase Ni-NTA
Content And Storage The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form Suspension
Column Type Affinity Column
7

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
8

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Promotions are available
Buffer Lysis Buffer
Shipping Condition Ambient
Form Liquid
Product Type Nuclear and Cytoplasmic Extraction Reagent
Reagent Type Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Product Line NE-PER™
9

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Promotions are available
EDGE
10

Thermo Scientific™ CaptureSelect™ IgG4 (Hu) Affinity Matrix

Thermo Scientific™ CaptureSelect™ IgG4 (Hu) Affinity Matrix has been specifically designed for the purification and isolation of human IgG4 from complex sources such as plasma, serum, or cell culture supernatants.

Promotions are available
Particle Size 65 ±10 μm
Ligand Type IgG4 affinity ligand
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Type Affinity Matrix
Matrix Agarose-based, epoxide activated
Form Resin Slurry
Binding Capacity >15 g IgG4/L resin
Product Line CaptureSelect™
11

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Bulk Resin
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format Bottle
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Column Type Suspension
Concentration 50% suspension
12

IBA Lifesciences Strep-Tactin™ 4Flow™ High Capacity Cartridge

Strep-Tactin™ 4Flow™ high capacity cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
Particle Size 50 to 150 μm
Target Twin-Strep-Tag™ and Strep-tag™II
Format Pre-packed Cartridge
For Use With (Application) HPLC/FPLC
Support Type 4% agarose, crosslinked
14

Novus Biologicals™ Amintra (R) Desalting Spin Columns

Content And Storage 4°C
For Use With (Application) Flow Cytometry