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Restriction Enzymes

Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.
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1 – 8 of 8 results
1

Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme BpiI (BbsI)
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Scarless Cloning
Compatible Buffer 10X Buffer G
Type IIS RE Yes
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
2

Thermo Scientific™ FastDigest Eco31I (IIs class)

The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Eco31I
Methylation Sensitivity CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive
Research Category Scarless Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE Yes
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
3

Thermo Scientific™ OliI (AleI) (10 U/μL)

The OliI (AleI) restriction enzyme recognizes CACNN^NNGTG sites and cuts best at 37°C in R buffer (Isoschizomers: AleI).

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme OliI (AleI)
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer R
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
4

Thermo Scientific™ Esp3I (BsmBI) (10 U/μL)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Esp3I (BsmBI)
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Scarless Cloning
Compatible Buffer 10X Buffer Tango
Type IIS RE Yes
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
5

Thermo Scientific™ MssI (PmeI) (5 U/μL)

The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme MssI (PmeI)
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer B
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 5 U/μL
6

Thermo Scientific™ Tango Buffer (10X)

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability.

Promotions are available
7

Thermo Scientific™ XhoI (10 U/μL)

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).

Concentration 10 U/μL
8

Thermo Scientific™ SmaI (10 U/μL)

Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).

Concentration 10 U/μL