Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.

Brand:  Invitrogen™ 12346086

Product Code. 10125142

Includes: AccuPrime™ Taq DNA Polymerase High Fidelity (40μL, at 5U/μL)) is supplied with: 50mM MgSO₄ (1mL), 10X AccuPrime™ PCR Buffer I (1mL), 10X AccuPrime™ PCR Buffer II (1mL)

Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600mM Tris-SO₄ (pH 8.9), 180mM (NH₄)₂SO₄, 20mM MgSO₄, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λDNA. Buffer II is optimized for genomic DNA.

Description

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

Features of AccuPrime Taq DNA Polymerase, High Fidelity

• High specificity and yield for robust PCR amplification
• 9-fold higher fidelity than Taq DNA polymerase alone
• Mimimal optimization steps, even with non-optimized primer sets
• Efficient amplification of targets over a broad size range up to 20 kb

How it works

Pyrococcus species GB-D polymerase is a proofreading enzyme that possesses a 3' to 5' exonuclease activity. Mixing this enzyme with Taq DNA Polymerase increases fidelity and allows amplification of simple and complex DNA templates over a large range of target sizes.

The Platinum antibody complexes with Taq DNA polymerase and inhibits activity at room temperature. Activity is restored after the initial denaturation step, providing an automatic hot start PCR.

The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: • AccuPrime Taq DNA Polymerase, High Fidelity (1 x 40 μL at 5 U/μL)
  • 10X AccuPrime PCR Buffer I (1 x 1 mL)
  • 10X AccuPrime PCR Buffer II (1 x 1 mL)
  • 50 mM MgSO₄ (1 x 1 mL)
  
  Store at -10°C to -30°C.
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Quantity: 200 rxns
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Hot Start: Built-In Hot Start
• Overhang: Mixed
• Size (Final Product): 20 kb or less

• Detection Method: Primer-probe
• Polymerase: AccuPrime Taq DNA Polymerase High Fidelity
• Product Type: DNA Polymerase
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 9X
• No. of Reactions: 200 Reactions
• Reaction Format: Separate Components

For Research Use Only. Not for use in diagnostic procedures.