Invitrogen™ Platinum™ Tfi DNA Polymerase

Platinum™ Tfi DNA Polymerase

Brand:  Invitrogen™ 73684050

Product Code. 10403643

Description

Platinum™ Tfi DNA Polymerase combines Platinum™ automated hot-start technology with a non-Taq, surfactant-free PCR enzyme derived from Thermus filiformis. PCR performance is comparable to Platinum™ Taq DNA polymerase in yield, specificity, fidelity, and robustness for targets less than 1 kb in length. In addition, Platinum™ Tfi is available in a 5'Exo (-) form for PCR applications that do not require the polymerase to have 5'exonuclease activity. With Platinum™ Tfi, you’ll:

–Improve PCR sensitivity and yield with an automated hot start

–Generate more available enzyme during the enzyme activation step for improved PCR robustness
–Amplify more DNA with less enzyme per reaction


Available for OEM: Unlike Platinum™ Taq DNA Polymerase, a customized version of Platinum™ Tfi DNA Polymerase can be made available for resale as a part of a PCR kit for basic research, diagnostics, or other applied fields. Please contact an Invitrogen representative for more information.

Applications: Amplification of DNA from complex genomic, viral, and plasmid templates.

Unit definition: One unit of Platinum™ Tfi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into DNA in 30 minutes at 74°C.

Contents: Platinum™ Tfi DNA Polymerase is supplied with a magnesium free 5X Platinum™ Tfi Reaction Buffer and a separate vial 50 mM MgCl₂ .

Contents and Storage:
Platinum™ Tfi DNA Polymerase is supplied with 5X Platinum™ Tfi Reaction Buffer and 50 mM Magnesium Chloride. Store at -20°C (non-frost-free).

Specifications

• Content And Storage: Store in freezer (-5 to -30°C).
• Polymerase: Tfi DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 500 unit
• Fidelity (vs. Taq): 1 X
• Overhang: 3'-A
• Size (Final Product): 1 kb or less

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Line: Platinum™
• Shipping Condition: Dry Ice
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components

For Research Use Only. Not for use in diagnostic procedures.