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Real Time PCR Reagents and Kits

Real Time PCR Reagents and Kits

Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.

In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.

A typical RT-PCR kit includes the main components needed for the assay:

  • Buffer
  • Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
  • Reverse transcriptase reagent

 

The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:

    • Fluorogenic primers or labeled fluorogenic probes
    • Fluorescent dye

 

Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.

RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.

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1 – 15 of 33 results
1

Invitrogen™ ROX Reference Dye

ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.

2

SuperScript™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.

Promotions are available
EDGE
Polymerase Taq DNA Polymerase
Format Tube
Product Type qRT-PCR Kit
For Use With (Equipment) 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Sample Type RNA
Reaction Speed Fast or Standard
PCR Method 1-step RT-qPCR
Product Line SuperScript™
Detection Method Primer-probe
4

SYBR™ GreenER™ EXPRESS One-Step SYBR™ GreenER™ Kit, universal

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments.

Polymerase Taq DNA Polymerase
Content And Storage EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format Tube
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Sample Type RNA
Reaction Speed Fast or Standard
PCR Method 1-step RT-qPCR
Product Line SYBR™ GreenER™
Detection Method SYBR
5

Invitrogen™ EXPRESS One-Step SYBR™ GreenER™ Kit, with premixed ROX

Polymerase Taq DNA Polymerase
Content And Storage EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX premixed.

Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format Tube
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type RNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line SYBR™ GreenER™
Detection Method SYBR
6

SuperScript™, Platinum™, SYBR™ SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX

Combine a powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Content And Storage Store in freezer (-5 to -30°C).
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System
Sample Type RNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Platinum™, SYBR™, SuperScript™
Detection Method SYBR
7

Applied Biosystems™ TaqMan™ SARS-CoV-2, Flu A/B, RSV RNA Control

The TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control contains targets specific to the SARS-CoV-2, influenza A/B, and RSV genomic regions targeted by the TaqMan SARS-CoV-2, FluA/B RSV RT-PCR Assay Kit.

8

SuperScript™, Platinum™, SYBR™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Real Time PCR (qPCR)
PCR Method 1-step RT-qPCR
Product Line Platinum™, SYBR™, SuperScript™
Detection Method SYBR
9

SuperScript™ EXPRESS One-Step Superscript™ qRT-PCR Kit, with premixed ROX

Next-generation, real-time qRT-PCR reagents designed to complement fast-cycling instruments. Get the turnaround time you want with the data quality you need.

Polymerase Taq DNA Polymerase
Content And Storage EXPRESS One-Step SuperScript™ qRT-PCR SuperMixes are composed of 2 modules: an EXPRESS RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and an EXPRESS qPCR SuperMix module with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed stable for 6 months when properly stored.
Format Tube
Product Type qRT-PCR Kit
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type RNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line SuperScript™
Detection Method Primer-probe
10

Platinum™, ThermoScript™ Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System

Platinum Quantitative RT-PCR ThermoScript One-Step System is for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Content And Storage Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum™ Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO4 ). A vial of 50 mM MgSO4 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Product Type RT-PCR One-Step System
Sample Type RNA
Reaction Speed Standard
PCR Method 1-step RT-qPCR
Product Line Platinum™, ThermoScript™
Detection Method Primer-probe
11

Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR Starter Kit

The QuantStudio Absolute Q Digital PCR Starter Kit is designed to confirm the performance of the QuantStudio Absolute Q Digital PCR System either at the time of system installation or as part of future, user-defined system verification procedures.

12

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Pathogen Detection,Virus Detection
PCR Method 1-step RT-qPCR
Product Line Platinum™, SuperScript™
Detection Method Primer-probe
13

SuperScript™, Platinum™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX

One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression.

Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Pathogen Detection,Virus Detection
PCR Method 1-step RT-qPCR
Product Line Platinum™, SuperScript™
Detection Method Primer-probe