Thermo Scientific™ Imperial™ Protein Stain

Detect proteins in the low-nanogram range with this ready-to-use, in-gel, colorimetric stain formulated to deliver fast and sensitive SDS-PAGE staining.

Brand:  Thermo Scientific™ 24617

Product Code. 10698074

Description

Thermo Scientific™ Imperial Protein Stain is a unique, ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers fast and sensitive total protein staining performance for low nanogram-level detection of proteins in polyacrylamide electrophoresis gels.

Imperial Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The Imperial Protein Stain protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.

Highlights:

• Sensitive – detects less than 3ng protein per band with the enhanced protocol (3 hours)

• Fast – ready-to-use reagent detects less than 6ng protein per band in just 20 minutes

• Robust – highly consistent, reproducible protein staining technique

• High contrast – intense purple bands are easier to photograph or scan than typical coomassie blue stains

• Versatile – compatible with downstream mass spectrometry analysis and protein sequencing

• Convenient – water washes only; no acid-fixative or methanol destaining solutions required

• Stable – 1-year room-temperature stability ensures consistent performance and saves refrigerator space

• Flexible – adjust staining and washing times to meet time- and sensitivity-requirements

Specifications

• Content And Storage: Upon receipt store product at room temperature.
• Detection Method: Colorimetric
• Product Type: Protein Stain
• Quantity: 3 x 1 L

• Detection Location: In-Blot Detection, In-Gel Detection
• Label or Dye: Coomassie
• Product Line: Imperial™
• Target Molecule: Protein

Safety and Handling

Product Identifier

• Imperial Protein Stain

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Acetic acid, ethanol

For Research Use Only. Not for use in diagnostic procedures.