Gibco™ RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand:  Gibco™ 21875091

Product Code. 12004997

Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light.
  Shipping conditions: Ambient.
  Shelf life: 12 months from date of manufacture.
• Cell Type: Mammalian Cells
• Concentration: 1 X
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: Glutamine, Phenol Red, Sodium Bicarbonate
• Green Features: Sustainable packaging
• Osmolality: 260 - 310 mOsm/kg
• Product Line: Gibco™

• Culture Environment: CO₂
• Classification: Animal Origin-free
• Culture Type: Mammalian Cell Culture
• Product Type: RPMI 1640
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• pH: 7 to 7.4
• Quantity: 10 x 500 mL

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.