Protein Purification Reagents and Kits
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Protein Purification Reagents and Kits
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Filtered Search Results
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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
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Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |
Sartorius Sartoclear Dynamics™ Lab V Filtration Kit
Sartorius Sartoclear Dynamics™ Lab V Kits designed for the clarification and sterile filtration of mammalian cell culture with diatomaceous earth as filter aid.
Product Type | Vacuum Filtration Units |
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Thermo Scientific™ CaptureSelect™ IgG4 (Hu) Affinity Matrix
CaptureSelect IgG4 (Hu) Affinity Matrix has been specifically designed for the purification and isolation of human IgG4 from complex sources such as plasma, serum, or cell culture supernatants.
Particle Size | 65 ±10 μm |
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Ligand Type | IgG4 affinity ligand |
Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
Type | Affinity Matrix |
Matrix | Agarose-based, epoxide activated |
Form | Resin Slurry |
Binding Capacity | >15 g IgG4/L resin |
Product Line | CaptureSelect™ |
Thermo Scientific™ Pierce™ Glutathione Spin Columns, 1 mL
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
Thermo Scientific™ AminoLink™ Immobilization Kit
Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
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Novus Biologicals™ Chromata ChIP Histone H3 [ac Lys9, ac Lys14] Kit (ChIP)
Applications: Chromatin Immunoprecipitation
Content And Storage | 4°C |
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For Use With (Application) | Flow Cytometry |
Novus Biologicals™ Chromata ChIP Histone H3 [ac Lys4] Kit (ChIP)
Applications: Chromatin Immunoprecipitation
Cytiva PreScission™ Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
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Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
Type | Agarose |
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Stationary Phase | Ni-NTA |
Content And Storage | The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
Form | Suspension |
Column Type | Affinity Column |
Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit
Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.
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