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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Brand: Thermo Scientific™ 37621

155.65 GBP valid until 2025-12-31
Use promo code "25339" to get your promotional price.

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Product Code. 10371394

£171.00 / 100mL

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Description

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

The substrate is offered in several formats:
PNPP Substrate
• Powder Form

PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.

Specifications

Description	1-Step™ PNPP Substrate Solution
Quantity	100 mL
Product Line	1-Step™
Substrate Type	Alkaline Phosphatase
Shipping Condition	Ambient

Product Type	ELISA Substrate
Detection Method	Colorimetric
Substrate Properties	Chemical Substrate
Content And Storage	Upon receipt store product at 4°C.

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Documents

Scientific Resources

Application Notes

Tech Tip: Guide to enzyme substrates for ELISA applications File size: 53.4 kB

Product Information

Manuals

User Guide: 1-Step PNPP Version: Jan. 2015 File size: 32.1 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZI4450161	Certificate TypeCertificate of Analysis	Date15/10/2024	Catalog Number37621
Lot NumberZH4228523	Certificate TypeCertificate of Analysis	Date31/08/2024	Catalog Number37621
Lot NumberZH4228524	Certificate TypeCertificate of Analysis	Date31/08/2024	Catalog Number37621
Lot NumberZF4228522	Certificate TypeCertificate of Analysis	Date19/06/2024	Catalog Number37621
Lot NumberZF4228521	Certificate TypeCertificate of Analysis	Date19/06/2024	Catalog Number37621

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