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Protein Purification Reagents and Kits

Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 127 results
1

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Promotions are available
EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
2

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Promotions are available
EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
3

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Promotions are available
EDGE
4

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

Promotions are available
EDGE
5

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

Particle Size 75μm
Buffer Aqueous Buffer
Ligand Type N-benzyl-n-methyl Ethanolamine
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type HiTrap Capto adhere column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Easy screening and convenient process development
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
6

Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Promotions are available
EDGE
Particle Size 8.6μm
Ligand Type Cross-linked agarose
Content And Storage 4° to 30° in 20% ethanol
pH Range 3 to 12
Column Type Gel Filtration Column
Material Borosilicate glass
Max. Pressure 435 psi
7

Cytiva Glutathione Sepharose™ 4B Media

Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.

Particle Size 90 μm
pH Range 4 to 13
Material Glutathione Sepharose 4 B
8

Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Particle Size 90μm
Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Flow Rate 150 to 600cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type Ni Sepharose excel chromato graphy media
dimMatrix Ni-Sepharose
Support Type 6% Highly Cross-linked Agarose
9

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

Promotions are available
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
10

Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Particle Size 90μm
Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Flow Rate 600 cm/hr.
Height (Metric) 250mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type Ni Sepharose 6 FF chromatography media
dimMatrix Ni-Sepharose
Support Type 6% Cross-linked Agarose
Max. Pressure 1 bar
Height (English) 9.84 in.
11

Cytiva HiTrap Capto MMC Columns

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Promotions are available
Particle Size 75μm
Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type HiTrap Capto MMC column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Bind Proteins at the conductivity of the feed material and to solve specific purification challenges
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
12

Cytiva MBPTrap™ HP Prepacked Columns

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 2° to 8°C
Bed Volume 1mL
pH Range >7
Column Type Ion Exchange Column
Material Polypropylene
For Use With (Application) Purification of MBP tagged Proteins
Max. Pressure 3 bar
Particle Size 34μm
Ligand Type Dextrin
Product Type Dextrin Sepharose HP prepacked column
Support Type 6% Highly Cross-linked Agarose
Height (English) 0.98 in.
13

Cytiva HiScreen Prepacked HIC Column, Capto Butyl

Intended for initial and intermediate step purifications that require a medium with low to medium hydrophobicity.

14

Cytiva GSTrap™ FF Columns: 7 x 25mm

Purifies GST-tagged proteins in one step

Promotions are available
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.27 in.
Bed Volume 1 mL
Description GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 3 to 12
Diameter (Metric) Inner 7 mm
Material Polypropylene
For Use With (Application) For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract
Particle Size 90 μm
Ligand Type Glutathione and 10 carbon linker arm
Flow Rate <4 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
15

Cytiva HiTrap™ Protein L Prepacked Columns

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

Particle Size 85μm
Ligand Type Recombinant Protein L (E. coli), mammalian free
Flow Rate <500 cm/hr.
Height (Metric) 20mm
Content And Storage 2° to 8°C
pH Range 2 to 10
Product Type HiTrap Protein L column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs
Support Type Highly Cross-linked Agarose
Max. Pressure 5 bar
Height (English) 0.78 in.