Protein Purification Reagents and Kits
![](https://assets.fishersci.com/TFS-Assets/CCG/product-images/protein-purification-reagents-and-kits-header-image.jpg-250.jpg)
Protein Purification Reagents and Kits
- (27)
- (13)
- (22)
- (6)
- (3)
- (4)
- (3)
- (3)
- (44)
- (4)
- (5)
- (4)
- (2)
- (4)
- (1)
- (1)
- (2)
- (2)
- (10)
- (2)
- (1)
- (1)
- (10)
- (1)
- (3)
- (26)
- (2)
- (1)
- (2)
- (6)
- (3)
- (5)
- (2)
- (1)
- (3)
- (1)
- (1)
- (1)
- (4)
- (3)
- (1)
- (2)
- (9)
- (2)
- (1)
- (1)
- (8)
- (9)
- (1)
- (3)
- (11)
- (1)
- (1)
- (1)
- (1)
Filtered Search Results
![](/catalog/search/static/resources/images/icons/back_to_top_icon.png)
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Flow Rate | <20 mL/min. |
---|---|
Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Particle Size | 75μm |
---|---|
Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva™ HiTrap™ Chromatography Columns with MabSelect™ VL Protein L Resin
Protein L affinity chromatography columns for capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain.
Height (Metric) | 25 mm |
---|---|
Column Type | Affinity Column |
For Use With (Application) | Antibody Affinity Chromatography |
Max. Pressure | 5 bar |
Height (English) | 1 in. |
Cytiva HiTrap™ Protein L Prepacked Columns
For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs
Particle Size | 85μm |
---|---|
Ligand Type | Recombinant Protein L (E. coli), mammalian free |
Flow Rate | <500cm/hr. |
Height (Metric) | 20mm |
Content And Storage | 2° to 8°C |
pH Range | 2 to 10 |
Product Type | HiTrap Protein L column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs |
Support Type | Highly Cross-linked Agarose |
Max. Pressure | 5 bar |
Height (English) | 0.78 in. |
Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Particle Size | 45 to 165 μm |
---|---|
Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Cytiva StrepTrap XT Chromatography Column
StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Height (Metric) | 25 mm |
---|---|
Column Type | Affinity Column |
For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™ |
Cytiva His SpinTrap™ Spin Columns
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.
Type | Spin Column Kit |
---|---|
Content And Storage | 4°C to 30°C |
Description | His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Type | Pre-packed Column |
---|---|
Content And Storage | 4°C to 30°C |
Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 2 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Particle Size | 34μm |
---|---|
Buffer | 6M Guanidine Hydrochloride, 8M Urea |
Ligand Type | Iminodiacetic Acid |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 13 |
Product Type | HiTrap chelating HP column |
Column Type | Affinity Column |
For Use With (Application) | IMAC and subsequent purification of Polyhistidine tagged Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 3 bar |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Con A 4B Columns
For separation and purification of glycoproteins, polysaccharides and glycolipids
Particle Size | 45 to 165μm |
---|---|
Buffer | Aqueous Buffer |
Ligand Type | Concanavalin A (13mg Con A/mL drained medium) |
Height (Metric) | 25mm |
Content And Storage | 4° to 8°C |
pH Range | 4 to 9 |
Product Type | HiTrap Con A Sepharose 4B column |
Column Type | Ion Exchange Column |
Material | Polypropylene |
For Use With (Application) | Convenient separation and purification of Glycoproteins, Polysaccharides and Glycolipids |
Support Type | 4% Agarose |
Max. Pressure | 3 bar |
Height (English) | 0.98 in. |
Cytiva MBPTrap™ HP Prepacked Columns
Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.
Buffer | Aqueous Buffer |
---|---|
Height (Metric) | 25mm |
Content And Storage | 2° to 8°C |
Bed Volume | 1mL |
pH Range | >7 |
Column Type | Ion Exchange Column |
Material | Polypropylene |
For Use With (Application) | Purification of MBP tagged Proteins |
Max. Pressure | 3 bar |
Particle Size | 34μm |
Ligand Type | Dextrin |
Product Type | Dextrin Sepharose HP prepacked column |
Support Type | 6% Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiScreen™ Capto™ S ImpAct Prepacked Column
HiScreen Capto S ImpAct columns are prepacked with Capto S ImpAct cation exchange chromatography medium, and are part of our process development platform. The columns are an excellent choice for method optimization and parameter screening.
Cytiva GSTrap™ HP Columns
Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.
Particle Size | 34 μm |
---|---|
Ligand Type | Glutathione |
Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Description | GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
For Use With (Application) | Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins |
Support Type | 4% Highly Cross-linked Agarose |