General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.

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Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane

Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.

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Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)

Optimized, easy-to-use blocker and primary antibody diluent system

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Invitrogen™ WesternBreeze™ Wash Solution (16X)

Optimized solution of concentrated buffered saline containing detergent

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Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

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Specifications

Content And Storage	Upon receipt store at room temperature. Product shipped at ambient temperature.
Form	Liquid
Product Line	Restore™

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

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Specifications

Content And Storage	Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Form	Liquid
Product Line	Restore™

Thermo Scientific™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

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Invitrogen™ iBind™ Flex Solution Kit

Detection kit for use with iBind™ Flex Western System

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Specifications

No. of Reactions	10 midi blots or 20 mini blots
Product Type	Flex Solution Kit
For Use With (Equipment)	iBind Flex Western Device
Membrane Compatibility	PVDF, Nitrocellulose
Product Line	iBind™
Detection Method	Colorimetric, Chromogenic, Chemiluminescence

Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled

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Invitrogen™ Bolt™ Antioxidant

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

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Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

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Specifications

Shipping Condition	Dry Ice
Description	The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type	Protein Labeling Reagent
For Use With (Equipment)	Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility	PVDF, Nitrocellulose
Shelf Life	At least 2 years from receipt
Run Time	10 minutes
For Use With (Application)	Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Product Line	Invitrogen™

Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels

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Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.